APEX1 anticorps

N° du produit ABIN6654475

L’anticorps Souris Monoclonal anti-APEX1 a été validé pour WB, IF, IHC (p), ICC et FACS. Il convient pour détecter APEX1 dans des échantillons de Humain.

Aperçu rapide pour APEX1 anticorps (ABIN6654475)

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APEX1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: CPTC-APEX1-2

Détail du produit anti-APEX1 anticorps

Purification: Purified

Pureté: Protein G affinity chromatography

Immunogène: Recombinant full-length human protein was used as the immunogen for the APE1 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the APE1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,IF/ICC: 1-2 μg/mL,FACS (intracellular): 1-2 μg/10^6 cells in 0.1ml,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the APE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur APEX1

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APE1 / APEX1

Sujet: APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus